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43-137 (0.01ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-137 (0.01ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

ACAT1 Antibody

CATALOG NUMBER: 43-137

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-DEEYKRVDFSKVPK
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 40kDa band observed in Human, Mouse and Rat Liver lysates (calculated MW of 45.2kDa according to Human NP_000010.1 and 44.81kDa to Mouse NP_659033.1). Recommended concentration: 0.01-0.03ug/ml. Primary incubation was 1 hour.Immunofluorescence: Customer finds particulate cytoplasm staining in HeLa. This data is from a previous batch, not on sale.
Predicted Molecular Weight:
Approx 45kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ACAT1
Additional Names:
ACAT1, acetyl-Coenzyme A acetyltransferase 1, ACAT, MAT, T2, THIL, acetoacetyl Coenzyme A thiolase
Protein Accession Number:
NP_000010.1
Protein Gi Number:
4557237
Ncbi Gene Id Number:
38
Background Reference 1:
Hongo S, Watanabe T, Arita S, Kanome T, Kageyama H, Shioda S, Miyazaki A, Leptin modulates ACAT1 expression and cholesterol efflux from human macrophages.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-137

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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