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Western blot analysis of extracts of various cell lines, using ACAT2 antibody (23-237) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human colon carcinoma using ACAT2 antibody (23-237) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human ovary cancer using ACAT2 antibody (23-237) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using ACAT2 antibody (23-237) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat ovary using ACAT2 antibody (23-237) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using ACAT2 antibody (23-237) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

ACAT2 Antibody

CATALOG NUMBER: 23-237

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-97 of human ACAT2 (NP_005882.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
BT-474
Positive Control 2:
MCF7
Positive Control 3:
HeLa
Positive Control 4:
HepG2
Positive Control 5:
PC-12
Positive Control 6:
Mouse liver
Predicted Molecular Weight:
Observed: 41kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ACAT2
Additional Names:
ACAT2, ACTL
Ncbi Gene Id Number:
39
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-237

List Size:
100 uL

List Price:

$436.00

Datasheet
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