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Western blot analysis of ACCN2 Antibody in CEM cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with ACCN2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of ACCN2 Antibody in CEM cell line lysates (35ug/lane)

ACCN2 Antibody

CATALOG NUMBER: 63-864

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Mouse, Rat
Immunogen:
This ACCN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 500-526 amino acids from the C-terminal region of human ACCN2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
60 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ASIC1
Additional Names:
Acid-sensing ion channel 1, ASIC1, Amiloride-sensitive cation channel 2, neuronal, Brain sodium channel 2, BNaC2, ASIC1, ACCN2, BNAC2
Protein Accession Number:
P78348
Protein Gi Number:
296439456
Ncbi Gene Id Number:
41
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ACCN2 encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member is expressed in most if not all brain neurons, and it may be an ion channel subunit; however, its function as an ion channel remains unknown.
Background Reference 1:
Sherwood,T., et.al., J. Biol. Chem. 284 (41), 27899-27907 (2009)
Background Reference 2:
Kapoor,N., et.al., J. Biol. Chem. 284 (36), 24526-24541 (2009)
Background Reference 3:
Samways,D.S., et.al., Cell Calcium 45 (4), 319-325 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-864

List Size:
400 ul

List Price:

$495.00

Datasheet
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