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<strong>Figure 1 ELISA Validation </strong><br>Coating Antigen: immunogen peptide, 3217P, 10 μg/mL, incubate at 4 °C overnight.Detection Antibodies: SARS-CoV-2 Spike antibody, 3217-biotin or 3217, dilution: 0.5-1000 ng/mL, incubate at RT for 1 hr. 3217-biotin was detected by HRP-conjugated streptavidin at 1:5,000 and 3217 was detected by anti-rabbit HRP conjugated secondary antibodies at 1:10,000 , incubate at RT for 1 hr.
<strong>Figure 2 Immunohistochemistry Validation of ACE-2 in COVID-19 Patient Lung</strong><br> Immunohistochemical analysis of paraffin-embedded human testis tissue using anti- ACE2 antibody (3217-biotin, 5 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C, following by streptavidin-HRP conjugate at 20 μg/mL. Counter stained with Hematoxylin.
Figure 1 ELISA Validation
Coating Antigen: immunogen peptide, 3217P, 10 μg/mL, incubate at 4 °C overnight.Detection Antibodies: SARS-CoV-2 Spike antibody, 3217-biotin or 3217, dilution: 0.5-1000 ng/mL, incubate at RT for 1 hr. 3217-biotin was detected by HRP-conjugated streptavidin at 1:5,000 and 3217 was detected by anti-rabbit HRP conjugated secondary antibodies at 1:10,000 , incubate at RT for 1 hr.
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ACE2 Antibody (biotin)

CATALOG NUMBER: 3217-biotin

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Biotin
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (88%)
Immunogen:
Anti-ACE2 antibody (biotin) (3217) was raised against a peptide corresponding to 18 amino acids near the carboxy terminus of human ACE2.
The immunogen is located within the last 50 amino acids of ACE2.
Conjugate:
Biotin
Tested Applications:
ELISA, IHC
Application Note:
IHC: 2 μg/mL
Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested.
Specificity:
Anti-ACE2 has no cross response to ACE1.
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Positive Control 2:
Cat. No. 10-401 - Human Kidney Tissue Slide
Positive Control 3:
Cat. No. 1306 - Human Spleen Tissue Lysate
Positive Control 4:
Cat. No. 1313 - Human Testis Tissue Lysate
Predicted Molecular Weight:
Predicted: 93kD
Observed: 130 kD (7 N-linked glycosylation)
Isoforms:
Human ACE2 has 2 isoforms, including isoform 1 (805aa, 93kD) and isoform 2 (555aa, 64kD). Mouse ACE2 also has 2 isoforms, including isoform 1 (805aa, 92kD) and isoform 2 (353aa, 40kD). Rat ACE2 has one isoform (805aa, 93kD). 3215 can detect human, mouse and rat.
Purification:
ACE2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ACE2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ACE2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ACE2
Additional Names:
ACE2 Antibody: ACEH, Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, ACEH, SARS-CoV receptor, SARS-CoV-2 receptor
Protein Accession Number:
NP_068576
Protein Gi Number:
11225609
Ncbi Gene Id Number:
59272
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ACE2 Antibody: Angiotensin-converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology. In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently. ACE2 has also been shown to be a functional receptor of the SARS coronavirus. Homology modeling shows 2019-nCoV has a similar receptor-binding domain structure as SARS-CoV, which suggests COVID-19 (2019-nCoV) may use ACE2 as a receptor in humans for infection. The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function.
Background Reference 1:
Donoghue et al. Circ. Res. 2000;87:1-9.
Background Reference 2:
Tipnis et al. J Biol. Chem. 2000;275:33238-43.
Background Reference 3:
Li et al. Nature 2003;426:450-4.
Background Reference 4:
Lu et al. The Lancet 2020 (published online).
Background Reference 5:
Crackower et al. Nature 2002;417:822-8.
Citation 1:
Wei, et al.Genome-wide CRISPR Screens Reveal Host Factors Critical for SARS-CoV-2 Infection. Cell. 2020 Oct 20;S0092-8674(20)31392-1. doi: 10.1016/j.cell.2020.10.028.PMID: 33147444

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3217-biotin

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

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