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<strong>Figure 1 Western Blot Validation in Mouse and Rat Tissue Lysates</strong><br>Loading: 25 µg of lysates per lane.Antibodies: ACE2 antibody, 24-033, at 1:1000 dilution. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST.
<strong>Figure 2 Western Blot Validation in HepG2 Cells</strong><br>Loading: 25 µg of lysates per lane. Antibodies: ACE2 antibody, 24-033, at 1:500 dilution. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST.
<strong>Figure 3 Immunohistochemistry Validation of ACE2 in Mouse Kidney Tissue</strong><br> Immunohistochemistry of paraffin-embedded mouse kidney tissue using ACE2 antibody, 24-033, at 1:100 dilution (40x lens).
Figure 1 Western Blot Validation in Mouse and Rat Tissue Lysates
Loading: 25 µg of lysates per lane.Antibodies: ACE2 antibody, 24-033, at 1:1000 dilution. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST.
Recombinant

ACE2 Antibody, Monoclonal

CATALOG NUMBER: 24-033

Clonality:
Recombinant Monoclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Virus
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Virus
Immunogen:
Synthetic Peptide
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000, IHC: 1:50 - 1:200
Purification:
Affinity purification
Clonality:
Recombinant Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH 7.3
Concentration:
batch dependent
Storage Conditions:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Ncbi Official Symbol:
ACE2
Additional Names:
ACEH, Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, ACEH, SARS-CoV receptor, SARS-CoV-2 receptor
Protein Accession Number:
Q9BYF1
Ncbi Gene Id Number:
59272
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008]

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

24-033

List Size:
100 ul

List Price:

$540.00

Datasheet
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