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Western blot analysis in Jurkat cell line lysates (35ug/lane).
Western blot analysis in Jurkat cell line lysates (35ug/lane).

ACER1 Antibody

CATALOG NUMBER: 60-034

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ACER1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-263 amino acids from the C-terminal region of human ACER1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
31 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ACER1
Additional Names:
Alkaline ceramidase 1, AlkCDase 1, Alkaline CDase 1, Acylsphingosine deacylase 3, N-acylsphingosine amidohydrolase 3, ACER1, ASAH3
Protein Accession Number:
Q8TDN7
Protein Gi Number:
74715919
Ncbi Gene Id Number:
125981
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Ceramides are synthesized during epidermal differentiationand accumulate within the interstices of the stratum corneum, wherethey represent critical components of the epidermal permeabilitybarrier. Excess cellular ceramide can trigger antimitogenic signalsand induce apoptosis, and the ceramide metabolites sphingosine andsphingosine-1-phosphate (S1P) are important bioregulatorymolecules. Ceramide hydrolysis in the nucleated cell layersregulates keratinocyte proliferation and apoptosis in response toexternal stress. Ceramide hydrolysis also occurs at the stratumcorneum, releasing free sphingoid base that functions as anendogenous antimicrobial agent. ACER1 is highly expressed inepidermis and catalyzes the hydrolysis of very long chain ceramidesto generate sphingosine (Houben et al., 2006 [PubMed 16477081]; Sunet al., 2008 [PubMed 17713573]).
Background Reference 1:
Sun, W., et al. J. Invest. Dermatol. 128(2):389-397(2008)
Background Reference 2:
Toulza, E., et al. Genome Biol.(6), R107 (2007) :
Background Reference 3:
Houben, E., et al. J. Lipid Res. 47(5):1063-1070(2006)
Background Reference 4:
Mao, C., et al. J. Biol. Chem. 278(33):31184-31191(2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-034

List Size:
400 ul

List Price:

$495.00

Datasheet
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