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Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2A-K9 antibody (13-886).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded mouse colon using Acetyl-Histone H2A-K9 antibody (13-886) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human mammary cancer using Acetyl-Histone H2A-K9 antibody (13-886) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using Acetyl-Histone H2A-K9 antibody (13-886) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using Acetyl-Histone H2A-K9 antibody (13-886) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using Acetyl-Histone H2A-K9 antibody (13-886) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse thyroid using Acetyl-Histone H2A-K9 antibody (13-886) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using Acetyl-Histone H2A-K9 antibody (13-886) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using Acetyl-Histone H2A-K9 antibody (13-886).
Immunofluorescence analysis of CRC cells using HK1 antibody (13-849).
Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2A-K9 antibody (13-886).
Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2A-K9 antibody (13-886).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Acetyl-Histone H2A-K9 mAb

CATALOG NUMBER: 13-886

Clonality:
Monoclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A specific peptide of human Acetyl-Histone H2A-K9
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:500 - 1:1000
IF: 1:100 - 1:500
Positive Control 1:
HeLa treated with Trichostatin A
Positive Control 2:
Untreated
Predicted Molecular Weight:
Observed: 14kDa
Purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
H2A K9ac
Ncbi Gene Id Number:
3012;8335
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-886

List Size:
50 uL

List Price:

$335.00

Out of stock

Datasheet
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