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Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2B-K12 antibody (16-140) at 1:1000 dilution.HeLa cells were treated by TSA (1 uM) at 37 for 18 hours.C2C12 cells were treated by TSA (1 uM) at 37 for 18 hours.C6 cells were treated by TSA (1 uM) at 37 for 18 hours.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% BSA.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human colon using Acetyl-Histone H2B-K12 antibody (16-140) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat ovary using Acetyl-Histone H2B-K12 antibody (16-140) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using Acetyl-Histone H2B-K12 antibody (16-140) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of C6 cells using Acetyl-Histone H2B-K12 antibody (16-140) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37 for 18 hours. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2B-K12 antibody (16-140) at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37 for 18 hours. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H2B-K12 antibody (16-140) at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37 for 18 hours. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2B-K12 antibody (16-140) at 1:1000 dilution.HeLa cells were treated by TSA (1 uM) at 37 for 18 hours.C2C12 cells were treated by TSA (1 uM) at 37 for 18 hours.C6 cells were treated by TSA (1 uM) at 37 for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 1s.

Acetyl-Histone H2B-K12 pAb

CATALOG NUMBER: 16-140

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic acetylated peptide around K12 of human Histone H2B (NP_003519.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
HeLa
Positive Control 2:
C2C12
Positive Control 3:
C6
Predicted Molecular Weight:
Observed: 14kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
H2B K12ac
Additional Names:
H2B, H2BQ, GL105, H2B.1, H2BFQ, H2BGL105
Ncbi Gene Id Number:
8349
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-dependent histone that is a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. The protein has antibacterial and antifungal antimicrobial activity.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-140

List Size:
100 uL

List Price:

$540.00

Datasheet
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