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Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2B-K15 antibody (16-143) at 1:1000 dilution.HeLa cells were treated by TSA (1 uM) at 37 for 18 hours.NIH/3T3 cells were treated by TSA (1 uM) at 37 for 18 hours.C6 cells were treated by TSA (1 uM) at 37 for 18 hours.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% BSA.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat lung using H2B K15ac antibody (16-143) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using H2B K15ac antibody (16-143) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using H2B K15ac antibody (16-143) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using H2B K15ac antibody (16-143) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using Acetyl-Histone H2B-K15 antibody (16-143) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37 for 18 hours. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2B-K15 antibody (16-143) at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37 for 18 hours. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H2B-K15 antibody (16-143) at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37 for 18 hours. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2B-K15 antibody (16-143) at 1:1000 dilution.HeLa cells were treated by TSA (1 uM) at 37 for 18 hours.NIH/3T3 cells were treated by TSA (1 uM) at 37 for 18 hours.C6 cells were treated by TSA (1 uM) at 37 for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 10s.

Acetyl-Histone H2B-K15 pAb

CATALOG NUMBER: 16-143

Clonality:
Polyclonal
Tested Applications:
IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
AA synthetic acetylated peptide corresponding to residues surrounding K15 of human Histone H2B
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100
IP: 1:50 - 1:200
Positive Control 1:
HeLa
Positive Control 2:
NIH/3T3
Positive Control 3:
C6
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
H2B K15ac
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-143

List Size:
100 uL

List Price:

$540.00

Datasheet
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