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Immunohistochemistry of paraffin-embedded human appendix using Acetyl-Histone H4-K12 antibody (15-437) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat ovary using Acetyl-Histone H4-K12 antibody (15-437) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using Acetyl-Histone H4-K12 antibody (15-437) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using Acetyl-Histone H4-K12 antibody (15-437) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of C6 cells using Acetyl-Histone H4-K12 antibody (15-437) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37 for 18 hours. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H4-K12 antibody (15-437) at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37 for 18 hours. Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded human appendix using Acetyl-Histone H4-K12 antibody (15-437) at dilution of 1:200 (40x lens).

Acetyl-Histone H4-K12 pAb

CATALOG NUMBER: 15-437

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
A synthetic acetylated peptide around K12 of human Histone H4 (NP_001029249.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
H4K12ac
Additional Names:
H4/A, H4FA, HIST1H4B, H4/I, H4FI, HIST1H4C, H4/G, H4FG, HIST1H4D, H4/B, H4FB, HIST1H4E, H4/J, H4FJ, HIST1H4F, H4/C, H4FC, HIST1H4H, H4/H, H4FH, HIST1H4I, H4/M, H4FM, HIST1H4J, H4/E, H4FE, HIST1H4K, H4/D, H4FD, HIST1H4L, H4/K, H4FK, HIST2H4A, H4/N, H4F2, H4FN, HIST2H4, HIST2H4B, H4/O, H4FO, HIST4H4, Histone H4H4FA
Ncbi Gene Id Number:
8366
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-437

List Size:
100 uL

List Price:

$540.00

Datasheet
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