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Western blot analysis of lysates from HeLa cells using Acetyl-Histone H4-K12 Rabbit mAb (A23683) at 1:1000 dilution incubated overnight at 4℃. HeLa cells were treated with TSA (1 μM) at 37℃ for 18 hours after serum starvation overnight. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 10 s.
Western blot analysis of lysates from C6 cells using Acetyl-Histone H4-K12 Rabbit mAb (A23683) at 1:1000 dilution incubated overnight at 4℃. C6 cells were treated with TSA (1 μM) at 37℃ for 18 hours after serum starvation overnight. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 10 s.
Immunohistochemistry analysis of paraffin-embedded Human colon using Acetyl-Histone H4-K12 Rabbit mAb (A23683) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse spleen using Acetyl-Histone H4-K12 Rabbit mAb (A23683) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunoprecipitation analysis of 600 μg extracts of HeLa cells using 5 μg Acetyl-Histone H4-K12 Rabbit mAb (A23683). Western blot was performed from the immunoprecipitate using Acetyl-Histone H4-K5 Rabbit mAb (A23080) at a dilition of 1:10000.
Chromatin immunoprecipitation analysis of extracts of Hela cells, using Acetyl-Histone H4-K12 antibody (A23683) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Western blot analysis of lysates from HeLa cells using Acetyl-Histone H4-K12 Rabbit mAb (A23683) at 1:1000 dilution incubated overnight at 4℃. HeLa cells were treated with TSA (1 μM) at 37℃ for 18 hours after serum starvation overnight. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 10 s.

Acetyl-Histone H4-K12 Rabbit Recombinant Antibody

CATALOG NUMBER: 82-706

Clonality:
Monoclonal
Tested Applications:
ChIP, ELISA, IHC-P, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Other (Wide Range Predicted), Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Other (Wide Range Predicted), Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ChIP, ELISA, IHC-P, IP, WB
Application Note:
WB,1:1000 - 1:3000, IHC-P,1:500 - 1:1000, IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements., ChIP,5μg antibody for 5μg-10μg of Chromatin
Positive Control 1:
C6 treated with TSA, HeLa treated with TSA
Predicted Molecular Weight:
11 kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
Histone H4
Additional Names:
H4/I; H4C1; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4FI; H4-16; H4C11; H4C12; H4C13; H4C14; H4C15; H4C16; HIST1H4B; Acetyl-Histone H4-K12
Protein Accession Number:
P62805
Ncbi Gene Id Number:
8359/8370
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-706

List Size:
100 uL

List Price:

$540.00

Datasheet
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