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Western blot analysis in A375 cell line lysates (35ug/lane).
ACF Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in A375 cell line lysates (35ug/lane).

ACF Antibody

CATALOG NUMBER: 55-326

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ACF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 394-423 amino acids from the C-terminal region of human ACF.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
65 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
A1CF
Additional Names:
APOBEC1 complementation factor, APOBEC1-stimulating protein, A1CF, ACF, ASP {ECO:0000312, EMBL:CAB947541}
Protein Accession Number:
Q9NQ94
Protein Gi Number:
74761651
Ncbi Gene Id Number:
29974
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Mammalian apolipoprotein B mRNA undergoes site-specific Cto U deamination, which is mediated by a multi-component enzymecomplex containing a minimal core composed of APOBEC-1 and acomplementation factor encoded by this gene. The gene product hasthree non-identical RNA recognition motifs and belongs to the hnRNPR family of RNA-binding proteins. It has been proposed that thiscomplementation factor functions as an RNA-binding subunit anddocks APOBEC-1 to deaminate the upstream cytidine. Studies suggestthat the protein may also be involved in other RNA editing or RNAprocessing events.
Background Reference 1:
Galloway, C.A., et al. Biochem. Biophys. Res. Commun. 391(1):659-663(2010)
Background Reference 2:
Blanc, V., et al. Mol. Cell. Biol. 25(16):7260-7269(2005)
Background Reference 3:
Deloukas, P., et al. Nature 429(6990):375-381(2004)
Background Reference 4:
Xie, K., et al. Proc. Natl. Acad. Sci. U.S.A. 101(21):8114-8119(2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-326

List Size:
400 ul

List Price:

$495.00

Datasheet
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