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<strong>Figure 1. Immunofluorescence Validation of Acinus In HeLa Cells </strong><br> Immunofluorescent analysis of methanol-fixed HeLa cells labeling Acinus with 2219 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.
Western blot analysis of Acinus in K562 whole cell lysate with Acinus antibody (IN) at 1 μg/mL.
Immunocytochemistry of Acinus in K562 cells with Acinus antibody at 5 μg/mL.
Immunofluorescence of Acinus in K562 cells with Acinus antibody at 20 μg/mL.
Immunofluorescence of Acinus in K562 cells with Acinus antibody at 20 μg/ml.<br><br>Green: Acinus Antibody (2219) <br> Blue: DAPI staining
Figure 1. Immunofluorescence Validation of Acinus In HeLa Cells
Immunofluorescent analysis of methanol-fixed HeLa cells labeling Acinus with 2219 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.
Trial Size

Acinus Antibody

CATALOG NUMBER: 2219

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (93%)
Immunogen:
Acinus antibody was raised against a 35 amino acid peptide near the center of human Acinus.
The immunogen is located within amino acids 760 - 810 of Acinus.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
Acinus antibody can be used for detection of Acinus by Western blot at 1 μg/mL. A 220 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Positive Control 2:
Cat. No. 17-004 - K-562 Cell Slide
Predicted Molecular Weight:
220 kDa
Purification:
Acinus Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Acinus Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Storage Conditions:
Acinus antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ACIN1
Additional Names:
Acinus Antibody: ACN, ACINUS, fSAP152, KIAA0670, Apoptotic chromatin condensation inducer in the nucleus, Acinus
Protein Accession Number:
AAD56724
Protein Gi Number:
5931959
Ncbi Gene Id Number:
22985
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Acinus Antibody: Chromatin condensation and nuclear fragmentation (CCNF) is the hallmark of apoptosis. CCNF is triggered by the activation of members of caspase family, caspase activated DNase (CAD/DFF40), and several novel proteins including AIF and CIDE. A new inducer of chromatin condensation was recently identified and designated Acinus (for apoptotic chromatin condensation inducer in the nucleus). Acinus is cleaved by caspase-3 and an additional unknown protease generating a small active peptide p17, which causes chromatin condensation in vitro when it is added to purified nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse and designated AcinusL, AcinusS and AcinusS'.
Background Reference 1:
Zamzami N, Kroemer G. Condensed matter in cell death. Nature 1999 ;401:127-8.
Background Reference 2:
Sahara S, Aoto M, Eguchi Y, Imamoto N, Yoneda Y, Tsujimoto Y. Acinus is a caspase-3-activated protein required for apoptotic chromatin condensation. Nature 1999 401:168-73.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2219

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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