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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the ACK1 antibody detected the ACK1 protein (arrow).
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the ACK1 antibody detected the ACK1 protein (arrow).

ACK1 Antibody

CATALOG NUMBER: 57-363

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This ACK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 265-290 amino acids from the Central region of human ACK1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
115 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TNK2
Additional Names:
Activated CDC42 kinase 1, ACK-1, Tyrosine kinase non-receptor protein 2, TNK2, ACK1
Protein Accession Number:
Q07912
Protein Gi Number:
229462980
Ncbi Gene Id Number:
10188
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a tyrosine kinase that binds Cdc42Hs inits GTP-bound form and inhibits both the intrinsic andGTPase-activating protein (GAP)-stimulated GTPase activity ofCdc42Hs. This binding is mediated by a unique sequence of 47 aminoacids C-terminal to an SH3 domain. The protein may be involved in aregulatory mechanism that sustains the GTP-bound active form ofCdc42Hs and which is directly linked to a tyrosine phosphorylationsignal transduction pathway. Several alternatively splicedtranscript variants have been identified from this gene, but thefull-length nature of only two transcript variants has beendetermined.
Background Reference 1:
Mahajan, K., et al. J. Cell. Physiol. 224(2):327-333(2010)
Background Reference 2:
Chua, B.T., et al. Mol Oncol 4(4):323-334(2010)
Background Reference 3:
Prieto-Echague, V., et al. J. Biol. Chem. 285(14):10605-10615(2010)
Background Reference 4:
Lin, Q., et al. Mol. Cell. Biol. 30(6):1541-1554(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-363

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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