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Western blot analysis of extracts from HepG2 cells treated with EGF using ACK1 (Phospho-Tyr284) Antibody. The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ACK1 (Phospho-Tyr284) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence staining of methanol-fixed A549 cells using ACK1 (Phospho-Tyr284) Antibody.
Western blot analysis of extracts from HepG2 cells treated with EGF using ACK1 (Phospho-Tyr284) Antibody. The lane on the right is treated with the antigen-specific peptide.

ACK1 (phospho Tyr284) Antibody

CATALOG NUMBER: 79-795

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
ACK1 (phospho Tyr284) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 284 (D-H-Y (p)-V-M) derived from Human ACK1.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
The antibody detects endogenous levels of ACK1 only when phosphorylated at tyrosine 284.
Predicted Molecular Weight:
120 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
TNK2
Additional Names:
ACK1, Activated p21cdc42Hs kinase, EC 2.7.10.2, kinase ACK1
Protein Accession Number:
NP_005772.3
Protein Gi Number:
229462980
Ncbi Gene Id Number:
10188
Background:
Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes.
Background Reference 1:
Manser E., Nature 363:364-367(1993).
Background Reference 2:
Ota T., Nat. Genet. 36:40-45(2004).
Background Reference 3:
Eisenmann K.M., Nat. Cell Biol. 1:507-513(1999)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-795

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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