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42-525 (0.01ug/ml) staining of Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-525 (3.8ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-525 (0.01ug/ml) staining of Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ACO2 Antibody

CATALOG NUMBER: 42-525

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow, Pig
Immunogen:
The immunogen for this antibody is: C-QHVDVSPTSQRLQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 90kDa band observed in Human Heart lysates (calculated MW of 85.4kDa according to NP_001089.1). Recommended concentration: 0.01-0.03ug/ml.Immunohistochemistry:In paraffin embedded Human Breast shows mitochondrial staining in the epithelial cells of the ducts. Recommended concentration, 3-5ug/ml.Immunofluorescence: Customer finds weak pixulate staining in the cytoplasm of HeLa
Positive Control 1:
Cat. No. 1301 - Human Heart Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ACO2
Additional Names:
ACO2, aconitase 2, mitochondrial, ACONM, MGC20605, MGC33908,aconitate hydratase, citrate hydro-lyase
Protein Accession Number:
NP_001089.1
Protein Gi Number:
4501867
Ncbi Gene Id Number:
50

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-525

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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