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Western blot analysis in HepG2 cell line lysates (35ug/lane).
ACOT11 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in HepG2 cell line lysates (35ug/lane).

ACOT11 Antibody

CATALOG NUMBER: 55-048

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ACOT11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 549-575 amino acids from the C-terminal region of human ACOT11.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
68 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ACOT11
Additional Names:
Acyl-coenzyme A thioesterase 11, Acyl-CoA thioesterase 11, 312-, Acyl-CoA thioester hydrolase 11, Adipose-associated thioesterase, Brown fat-inducible thioesterase, BFIT, ACOT11, BFIT, KIAA0707, THEA
Protein Accession Number:
Q8WXI4
Protein Gi Number:
21363000
Ncbi Gene Id Number:
26027
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the acyl-CoA thioesterasefamily which catalyse the conversion of activated fatty acids tothe corresponding non-esterified fatty acid and coenzyme A.Expression of a mouse homolog in brown adipose tissue is induced bylow temperatures and repressed by warm temperatures. Higher levelsof expression of the mouse homolog has been found inobesity-resistant mice compared with obesity-prone mice, suggestinga role of acyl-CoA thioesterase 11 in obesity. Alternative splicingresults in transcript variants.
Background Reference 1:
Kirkby, B., et al. Prog. Lipid Res. 49(4):366-377(2010)
Background Reference 2:
Hunt, M.C., et al. J. Lipid Res. 46(9):2029-2032(2005)
Background Reference 3:
Adams, S.H., et al. Biochem. J. 360 (PT 1), 135-142 (2001) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-048

List Size:
400 ul

List Price:

$495.00

Datasheet
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