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Western blot analysis of ACR Antibody in Jurkat cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human testis tissue reacted with ACR Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of ACR Antibody in Jurkat cell line lysates (35ug/lane)

ACR Antibody

CATALOG NUMBER: 63-771

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ACR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 387-416 amino acids from the C-terminal region of human ACR.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
46 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ACR
Additional Names:
Acrosin, Acrosin light chain, Acrosin heavy chain, ACR, ACRS
Protein Accession Number:
P10323
Protein Gi Number:
115502349
Ncbi Gene Id Number:
49
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ACR is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids.
Background Reference 1:
Dube,C., et.al., J. Androl. 26 (4), 519-528 (2005)
Background Reference 2:
Furlong,L.I., et.al., Fertil. Steril. 83 (6), 1791-1796 (2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-771

List Size:
400 ul

List Price:

$495.00

Datasheet
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