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Western blot analysis of ACSL1 expression in human liver tissue lysate with ACSL1 antibody at 0.25 μg/ml.
Immunofluorescence of ACSL1 in human lung tissue with ACSL1 antibody at 20 μg/mL.
Western blot analysis of ACSL1 expression in human liver tissue lysate with ACSL1 antibody at 0.25 μg/ml.
Trial Size

ACSL1 Antibody

CATALOG NUMBER: 7081

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Guinea pig: (88%)
Immunogen:
Rabbit polyclonal ACSL1 antibody was raised against an 18 amino acid peptide near the center of human ACSL1.
The immunogen is located within amino acids 240 - 290 of ACSL1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
ACSL1 antibody can be used for detection of ACSL1 by Western blot at 0.25 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least three isoforms of ACSL1 are known to exist; this antibody will detect all three isoforms.
Positive Control 1:
Cat. No. 1304 – Human Liver Tissue Lysate
Positive Control 2:
Cat. No. 10-101 - Human Lung Tissue Slide
Predicted Molecular Weight:
Predicted: 41, 58, 77 kDa
Observed: 45, 47 kDa 
Purification:
ACSL1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ACSL1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ACSL1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ACSL1
Additional Names:
ACSL1 Antibody: ACS1, LACS, FACL1, FACL2, LACS1, LACS2, Long-chain-fatty-acid--CoA ligase 1, Acyl-CoA synthetase 1, ACS1
Protein Accession Number:
NP_001986
Protein Gi Number:
40807491
Ncbi Gene Id Number:
2180
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ACSL1 Antibody: Long-chain acyl coenzyme A synthetase 1 (ACSL1) catalyzes the synthesis of acyl-CoA from long-chain fatty acids in an ATP-dependent manner. ACSL1 is a member of a family of long-chain acyl-CoA synthetases which differ in substrate preference, tissue expression, and subcellular localization. In mouse, ACSL1 is the major acyl-CoA enzyme in the heart, providing 60-90% of heart ATP. Loss of ACSL1 either globally or in heart ventricles resulted in impaired fatty acid oxidation, activation of the mammalian target of rapamycin (mTOR), and cardiac hypertrophy.
Background Reference 1:
Black PN and DiRusso CC. Transmembrane movement of exogenous long-chain fatty acids: proteins, enzymes, and vectorial esterification. Microbiol. Mol. Biol. Rev. 2003; 67:454-72.
Background Reference 2:
Coleman RA, Lewin TM, Van Horn CG, et al. Do acyl-CoA synthetases regulate fatty acid entry into synthetic versus degradative pathways? J. Nutr. 2002; 132:2123-6.
Background Reference 3:
Clark H, Carling D, and Saggerson D. Covalent activation of heart AMP-activated protein kinase in response to physiological concentrations of long-chain fatty acids. Eur. J. Biochem. 2004; 271:2215-24
Background Reference 4:
Ellis JM, Mentock SM, DePetrillo MA, et al. Mouse cardiac acyl Coenzyme A synthetase 1 deficiency impairs fatty acid oxidation and induces cardiac hypertrophy. Mol. Cell. Biol. 2011; 31:1252-62.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7081

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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