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Western blot analysis of extracts of various cell lines, using ACSL3 antibody (14-096) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human mammary cancer using ACSL3 antibody (14-096) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using ACSL3 antibody (14-096) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using ACSL3 antibody (14-096) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using ACSL3 antibody (14-096) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using ACSL3 antibody (14-096) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.

ACSL3 Antibody

CATALOG NUMBER: 14-096

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 42-300 of human ACSL3 (NP_004448.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
HeLa
Positive Control 2:
Mouse kidney
Predicted Molecular Weight:
Observed: 75kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ACSL3
Additional Names:
ACSL3, ACS3, FACL3, PRO2194
Ncbi Gene Id Number:
2181
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates. The amino acid sequence of this isozyme is 92% identical to that of rat homolog. Two transcript variants encoding the same protein have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-096

List Size:
100 uL

List Price:

$436.00

Datasheet
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