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Antibody used in WB on Human 293T at 0.2-1 ug/ml.
Antibody used in IHC on Human THP-1 at: 1:200.
Antibody used in IHC on Human THP-1 at: 1:200.
Antibody used in WB on Human 293T at 0.2-1 ug/ml.

ACSL3 Antibody

CATALOG NUMBER: 26-138

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ACSL3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ACSL3 antibody can be used for detection of ACSL3 by ELISA at 1:312500. ACSL3 antibody can be used for detection of ACSL3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
293T Cell Lysate
Predicted Molecular Weight:
80 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store ACSL3 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
ACSL3
Additional Names:
ACSL3, ACS3, FACL3, PRO2194
Protein Accession Number:
NP_004448
Protein Gi Number:
42794752
Ncbi Gene Id Number:
2181
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ACSL3 is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates. The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates. The amino acid sequence of this isozyme is 92% identical to that of rat homolog. Two transcript variants encoding the same protein have been found for this gene.
Background Reference 1:
Yao, H. (2008) J. Biol. Chem. 283 (2), 849-854.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

26-138

List Size:
100 ul

List Price:

$519.00

Datasheet
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