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Western blot analysis in 293 cell line lysates (35ug/lane).
Western blot analysis in 293 cell line lysates (35ug/lane).

ACSL3 Antibody

CATALOG NUMBER: 58-620

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This ACSL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 528-556 amino acids from the C-terminal region of human ACSL3.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
80 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ACSL3
Additional Names:
Long-chain-fatty-acid--CoA ligase 3, Long-chain acyl-CoA synthetase 3, LACS 3, ACSL3, ACS3, FACL3, LACS3
Protein Accession Number:
O95573
Ncbi Gene Id Number:
2181
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is an isozyme of thelong-chain fatty-acid-coenzyme A ligase family. Although differingin substrate specificity, subcellular localization, and tissuedistribution, all isozymes of this family convert free long-chainfatty acids into fatty acyl-CoA esters, and thereby play a key rolein lipid biosynthesis and fatty acid degradation. This isozyme ishighly expressed in brain, and preferentially utilizes myristate,arachidonate, and eicosapentaenoate as substrates. The amino acidsequence of this isozyme is 92% identical to that of rat homolog.Two transcript variants encoding the same protein have been foundfor this gene.
Background Reference 1:
Weedon-Fekjaer, M.S., et al. J. Lipid Res. 51(7):1886-1896(2010)
Background Reference 2:
Cao, A., et al. J. Biol. Chem. 285(22):16664-16674(2010)
Background Reference 3:
Perera, F., et al. PLoS ONE(2), E4488 (2009) :
Background Reference 4:
Yao, H., et al. J. Biol. Chem. 283(2):849-854(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-620

List Size:
400 ul

List Price:

$495.00

Datasheet
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