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45-197 (1ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-197 (5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-197 (1ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ACSL5 Antibody

CATALOG NUMBER: 45-197

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-RTQIDSLYEHIQD
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 75kDa band observed in Human Spleen lysates (calculated MW of 76.0kDa according to NP_976313.1and NP_976314.1). Recommended concentration: 1-3ug/ml.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognise isoform a (NP_057318.2) and isoform b (NP_976313.1 and NP_976314.1).
Positive Control 1:
Cat. No. 1306 - Human Spleen Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ACSL5
Additional Names:
ACSL5, acyl-CoA synthetase long-chain family member 5, HGNC:16526, ACS2, ACS5, FACL5, fatty-acid-Coenzyme A ligase, long-chain 5, long-chain acyl-CoA synthetase 5, long-chain fatty acid coenzyme A ligase 5
Protein Accession Number:
NP_057318.2,NP_976313.1,NP_976314.1
Protein Gi Number:
42794756
Ncbi Gene Id Number:
51703
Background Reference 1:
Obermuller N, Keith M, Kopitz J, Autschbach F, Schirmacher P, Gassler N. Coeliac disease is associated with impaired expression of acyl-CoA-synthetase 5. Int J Colorectal Dis. 2005 Apr 5;

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-197

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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