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Western blot analysis in A549,RD cell line and human placenta lysates (35ug/lane).This demonstrates the ACTA1/alpha -actin antibody detected the ACTA1/alpha -actin protein (arrow).
ACTA1/alpha -actin Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of ACTA1/alpha -actin Antibody with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in A549,RD cell line and human placenta lysates (35ug/lane).This demonstrates the ACTA1/alpha -actin antibody detected the ACTA1/alpha -actin protein (arrow).

ACTA1/Alpha-actin Antibody

CATALOG NUMBER: 57-981

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat, Rabbit, Pig, Chicken, Xenopus, Bovine
Immunogen:
This ACTA1/Alpha-actin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-375 amino acids from the C-terminal region of human ACTA1/Alpha-actin.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
42 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ACTA1
Additional Names:
Actin, alpha skeletal muscle, Alpha-actin-1, ACTA1, ACTA
Protein Accession Number:
P68133
Protein Gi Number:
61218043
Ncbi Gene Id Number:
58
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The product encoded by this gene belongs to the actinfamily of proteins, which are highly conserved proteins that play arole in cell motility, structure and integrity. Alpha, beta andgamma actin isoforms have been identified, with alpha actins beinga major constituent of the contractile apparatus, while beta andgamma actins are involved in the regulation of cell motility. Thisactin is an alpha actin that is found in skeletal muscle. Mutationsin this gene cause nemaline myopathy type 3, congenital myopathywith excess of thin myofilaments, congenital myopathy with cores,and congenital myopathy with fiber-type disproportion, diseasesthat lead to muscle fiber defects.
Background Reference 1:
Kim, E.Y., et al. Am. J. Physiol. Renal Physiol. 299 (3), F594-F604 (2010) :
Background Reference 2:
Haigh, S.E., et al. Neuromuscul. Disord. 20(6):363-374(2010)
Background Reference 3:
Yu, G., et al.Clin Neurosci 17(6):766-769(2010)
Background Reference 4:
Yu, C.H., et al. PLoS ONE(7), E11878 (2010) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-981

List Size:
400 ul

List Price:

$519.00

Datasheet
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