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Antibody staining ACTA1/alpha-actin in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Western blot analysis in human normal uterus and placenta,rat heart tissue lysates (35ug/lane).This demonstrates the ACTA1/alpha -actin antibody detected the ACTA1/alpha -actin protein (arrow).
Antibody staining ACTA1/alpha-actin in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

ACTA1/alpha-actin Antibody

CATALOG NUMBER: 59-237

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Homology:
Predicted species reactivity based on immunogen sequence: C. Elegans, Drosophila, Xenopus, Chicken, Bovine, Mouse, Rabbit, Zebrafish, Hamster, Monkey, Pig, Yeast
Immunogen:
This ACTA1/Alpha-actin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 189-217 amino acids from the C-terminal region of human ACTA1/Alpha-actin.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For IHC-P starting dilution is: 1:25
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
42 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ACTA1
Additional Names:
Actin, alpha skeletal muscle, Alpha-actin-1, ACTA1, ACTA
Protein Accession Number:
P68133
Protein Gi Number:
61218043
Ncbi Gene Id Number:
58
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
Background Reference 1:
Kim, E.Y., et al. Am. J. Physiol. Renal Physiol. 299 (3), F594-F604 (2010)Haigh, S.E., et al. Neuromuscul. Disord. 20(6):363-374(2010) Yu, G., et al.Clin Neurosci 17(6):766-769(2010) Yu, C.H., et al. PLoS ONE(7), E11878 (2010)Licastro, F., et al. Curr. Pharm. Des. 16(7):783-788(2010)

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-237

List Size:
400 ul

List Price:

$469.00

Out of stock

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