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45-198 (0.3ug/ml) staining of HeLa (A) and (0.5ug/ml) NIH3T3 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-198 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (
45-198 (3.5ug/ml) staining of paraffin embedded Human Lung. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining. <strong>This data is from a previous batch, not on sale.</strong>
45-198 Negative Control showing staining of paraffin embedded Human Lung, with no primary antibody. <strong>This data is from a previous batch, not on sale.</strong>
45-198 (0.1ug/ml) staining of Human (A), (0.3ug/ml) Mouse (B) and Rat (C) Duodenum lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-198 (0.3ug/ml) staining of HeLa (A) and (0.5ug/ml) NIH3T3 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Actin Antibody

CATALOG NUMBER: 45-198

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: EEEDSTALVC
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 45kDa band observed in Human, Mouse and Rat Duodenum lysates and in lysates of cell lines HeLa and NIH3T3, (calculated MW of 42kDa according to Human NP_001604, Mouse NP_031418.1 and Rat NP_112266.1). Recommended concentration: 0.1-1ug/ml. Primary incubation 1 hour at room temperature.
Specificity:
Variants NP_001604.1 and NP_001135417.1 encode the same protein.
Positive Control 1:
Cat. No. 1364 - Human Duodenum Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ACTA2
Additional Names:
ACTA2, actin, alpha 2, smooth muscle, aorta, OTTHUMP00000020042, ACTSA, AAT6, ACTSA, alpha 2 actin, alpha-cardiac actin, growth-inhibiting gene 46, smooth muscle alpha-actin,
Protein Accession Number:
NP_001604.1,NP_001135417.1
Protein Gi Number:
4501883
Ncbi Gene Id Number:
59
Background Reference 1:
Reddy S, Ozgur K, Lu M, Chang W, Mohan SR, Kumar CC, Ruley HE. Structure of the human smooth muscle alpha-actin gene. Analysis of a cDNA and 5' upstream region. J Biol Chem. 1990 Jan 25;265(3):1683-7.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-198

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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