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Western blot analysis of ACTB/ACTC Antibody in HL60 cell line lysates (35ug/lane).
Western blot analysis of ACTB/ACTC using rabbit polyclonal ACTB/ACTC Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACTB/ACTC gene (Lane 2).
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with ACTB/ACTC antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of ACTB/ACTC Antibody in HL60 cell line lysates (35ug/lane).

Actin Antibody

CATALOG NUMBER: 58-099

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: C. Elegans, Drosophila, Xenopus, Chicken, Bovine, Mouse, Rabbit, Rat, Zebrafish, Hamster, Monkey, Pig, Yeast
Immunogen:
This Actin (ACTB/ACTC) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 189-218 amino acids from the Central region of human Actin (ACTB/ACTC).
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
42 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ACTC1
Additional Names:
Actin, alpha cardiac muscle 1, Alpha-cardiac actin, ACTC1, ACTC
Protein Accession Number:
P68032
Protein Gi Number:
54036697
Ncbi Gene Id Number:
70
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. ACTB/ACTC are nonmuscle cytoskeletal actins and major constituents of the contractile apparatus. Defects in ACTB are a cause of juvenile-onset dystonia. Defects in ACTC have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC).
Background Reference 1:
Villebeck,L., Biochemistry 46 (44), 12639-12647 (2007)
Background Reference 2:
Avizienyte,E., Exp. Cell Res. 313 (15), 3175-3188 (2007)
Background Reference 3:
Bouldin,A.A., Muscle Nerve 35 (2), 254-258 (2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-099

List Size:
400 ul

List Price:

$495.00

Datasheet
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