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43-554 (0.3ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-554 (0.3ug/ml) staining of Mouse (A) and Rat (B) Spleen lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-554 (0.3ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ACTN1 Antibody

CATALOG NUMBER: 43-554

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-TPQEINGKWDHVRQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 100kDa band observed in Human, Mouse and Rat Spleen lysates (calculated MW of 103kDa according to NP_001093.1). Recommended concentration: 0.3-1ug/ml.An additional band of unknown identity was also consistently observed at 75kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Specificity:
This antibody is expected to recognize all reported isoforms (NP_001123476.1; NP_001093.1; NP_001123477.1).
Predicted Molecular Weight:
Approx 100kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ACTN1
Additional Names:
ACTN1, actinin, alpha 1, F-actin cross-linking protein, actinin 1 smooth muscle, alpha-actinin cytoskeletal isoform, alpha-actinin-1, non-muscle alpha-actinin-1
Protein Accession Number:
NP_001123476.1,NP_001093.1,NP_001123477.1
Protein Gi Number:
4501891
Ncbi Gene Id Number:
87
Background Reference 1:
Quick Q, Skalli O.Alpha-actinin 1 and alpha-actinin 4: contrasting roles in the survival, motility, and RhoA signaling of astrocytoma cells.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-554

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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