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Western blot analysis of extracts of various cell lines, using ACTR2 antibody (19-823) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using ACTR2 antibody (19-823) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human uterine cancer using ACTR2 antibody (19-823) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using ACTR2 antibody (19-823) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using ACTR2 antibody (19-823). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using ACTR2 antibody (19-823) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

ACTR2 Antibody

CATALOG NUMBER: 19-823

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human ACTR2 (NP_005713.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
THP-1
Positive Control 2:
293T
Positive Control 3:
HepG2
Positive Control 4:
HeLa
Positive Control 5:
C6
Positive Control 6:
Mouse spleen
Predicted Molecular Weight:
Observed: 45kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ACTR2
Additional Names:
ACTR2, ARP2
Ncbi Gene Id Number:
10097
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Two transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-823

List Size:
100 uL

List Price:

$436.00

Datasheet
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