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Western blot analysis in Hela,Jurkat cell line lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ACTR2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in Hela,Jurkat cell line lysates (35ug/lane).

ACTR2 Antibody

CATALOG NUMBER: 62-308

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Xenopus, Bovine, Chicken, Mouse, Rat
Immunogen:
This ACTR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 191-219 amino acids from the Central region of human ACTR2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
45 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ACTR2
Additional Names:
Actin-related protein 2, Actin-like protein 2, ACTR2, ARP2
Protein Accession Number:
P61160
Protein Gi Number:
47117648
Ncbi Gene Id Number:
10097
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ACTR2 is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion.
Background Reference 1:
Gonzalez,V., Cell Host Microbe(3), 259-272 (2009)
Background Reference 2:
Weisswange,I., Nature 458 (7234), 87-91 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-308

List Size:
400 ul

List Price:

$495.00

Datasheet
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