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43-502 (0.1ug/ml) staining of Mouse (A), Rat (B) and Pig (C) Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-502 (0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-502 (0.1ug/ml) staining of Mouse (A), Rat (B) and Pig (C) Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ADAM12 Antibody

CATALOG NUMBER: 43-502

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig, Rat
Homology:
Expected Species Reactivity based on sequence homology: Cow
Immunogen:
The immunogen for this antibody is: C-REFQRQGKDLEKVKQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 100kDa band observed in Human Heart lysates and approx 90+60kDa bands observed in Mouse, Rat and Pig Heart lysates (calculated MW of 99.5kDa according to NP_003465.3 and of 80.4kDa according to NP_067673.2). Recommended concentration: 0.1-0.3ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_003465.3; NP_067673.2).
Predicted Molecular Weight:
Approx 90 + 60kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ADAM12
Additional Names:
ADAM12, ADAM metallopeptidase domain 12, MCMP, MCMPMltna, MLTN, MLTNA, disintegrin and metalloproteinase domain-containing protein 12, meltrin-alpha
Protein Accession Number:
NP_003465.3,NP_067673.2
Protein Gi Number:
73747885
Ncbi Gene Id Number:
8038
Background Reference 1:
Ray BK, Dhar S, Shakya A, Ray A.Z-DNA-forming silencer in the first exon regulates human ADAM-12 gene expression.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-502

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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