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43-479 (0.1ug/ml) staining of Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-479 (5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-479 (0.1ug/ml) staining of Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ADAM19 Antibody

CATALOG NUMBER: 43-479

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: KIQCQSSEARPLESN
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 125kDa band observed in Human Spleen lysates (calculated MW of 101kDa according to NP_150377.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 5ug/ml.
Predicted Molecular Weight:
Approx 125kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ADAM19
Additional Names:
ADAM19, ADAM metallopeptidase domain 19, MADDAM, MLTNB, ADAM 19, a disintegrin and metalloproteinase domain 19 (meltrin beta), disintegrin and metalloproteinase domain-containing protein 19, meltrin beta, meltrin-beta, metalloprotease and disintegrin dendritic antigen marker
Protein Accession Number:
NP_150377.1
Protein Gi Number:
15451844
Ncbi Gene Id Number:
8728
Background Reference 1:
Yoshikawa A, Aizaki Y, Kusano K, Kishi F, Susumu T, Iida S, Ishiura S, Nishimura S, Shichiri M, Senbonmatsu T.The (pro)renin receptor is cleaved by ADAM19 in the Golgi leading to its secretion into extracellular space.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-479

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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