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Western blot analysis of extracts of various cell lines, using ADAM28 antibody (23-875) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Western blot analysis of extracts of various cell lines, using ADAM28 antibody (23-875) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

ADAM28 Antibody

CATALOG NUMBER: 23-875

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 199-440 of human ADAM28 (NP_055080.2).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:2000
Positive Control 1:
Raji
Positive Control 2:
HT-29
Positive Control 3:
A-549
Positive Control 4:
THP-1
Positive Control 5:
Mouse liver
Positive Control 6:
Rat liver
Predicted Molecular Weight:
Observed: 87kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ADAM28
Additional Names:
ADAM 28, MDC-L, MDCL, eMDC II, eMDCII, disintegrin and metalloproteinase domain-containing protein 28, epididymal metalloproteinase-like, disintegrin-like, and cysteine-rich protein II, epididymial metalloproteinase-like, disintegrin-like, and cysteine-rich protein II, epididymis secretory sperm binding protein, metalloproteinase-like, disintegrin-like, and cysteine-rich protein-L
Ncbi Gene Id Number:
10863
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene is a lymphocyte-expressed ADAM protein. This gene is present in a gene cluster with other members of the ADAM family on chromosome 8. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-875

List Size:
100 uL

List Price:

$436.00

Datasheet
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