Trial Size

ADAM29 Antibody

CATALOG NUMBER: 8183

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
ADAM29 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human ADAM29.
The immunogen is located within amino acids 710 - 760 of ADAM29.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
ADAM29 antibody can be used for detection of ADAM29 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
ADAM29 antibody is human specific. ADAM29 antibody is predicted to not cross-react with other ADAM family proteins.
Positive Control 1:
Cat. No. 1310 - Human Bladder Tissue Lysate
Positive Control 2:
Cat. No. 11-701 - Human Testis Tissue Slide
Predicted Molecular Weight:
Predicted: 76 kDa
Observed: 80 kDa
Purification:
ADAM29 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ADAM29 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ADAM29 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
ADAM29
Additional Names:
Disintegrin and metalloproteinase domain-containing protein 29, ADAM metallopeptidase domain 29, CT73, SVPH1
Protein Accession Number:
NP_055084
Protein Gi Number:
73765552
Ncbi Gene Id Number:
11086
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The ADAM (a disintegrin and metalloprotease) protein family plays a role in cell-cell and cell-matrix interface related processes, including fertilization, muscle fusion, secretion of TNFalpha (tumor necrosis factor alpha), and modulation of the neurogenic function of Notch and Delta (1-3). ADAM29 contains one disintegrin domain, one EGF-like domain and one peptidase M12B domain (2). It is highly expressed in testes (3) and may be involved in spermatogenesis and fertilization (4,5).
Background Reference 1:
Wang F, Xu R, Zhu P, et al. Preliminarily functional analysis of a cloned novel human gene ADAM29. Sci. China C. Life Sci. 2001; 44392-9.
Background Reference 2:
Xu R, Cai J, Xu T, et al. Molecular cloning and mapping of a novel ADAM gene (ADAM29) to human chromosome 4. Genomics 1999; 62:537-9.
Background Reference 3:
Cerretti DP, DuBose RF, Black RA, et al. Isolation of two novel metalloproteinase-disintegrin (ADAM) cDNAs that show testis-specific gene expression. Biochem. Biophys. Res. Commun. 1999; 263:810-5.
Background Reference 4:
Oppezzo P, Vasconcelos Y, Settegrana C, et al. The LPL/ADAM29 expression ratio is a novel prognosis indicator in chronic lymphocytic leukemia. Blood 2005; 106:650-7.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8183

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

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