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Western blot analysis of anti-ADAMTS13 Antibody in CEM cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma with ADAMTS13 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of anti-ADAMTS13 Antibody in CEM cell line lysates (35ug/lane)

ADAMTS13 Antibody

CATALOG NUMBER: 62-911

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ADAMTS13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 829-858 amino acids from the Central region of human ADAMTS13.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
154 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ADAMTS13
Additional Names:
A disintegrin and metalloproteinase with thrombospondin motifs 13, ADAM-TS 13, ADAM-TS13, ADAMTS-13, von Willebrand factor-cleaving protease, vWF-CP, vWF-cleaving protease, ADAMTS13, C9orf8
Protein Accession Number:
Q76LX8
Protein Gi Number:
74749836
Ncbi Gene Id Number:
11093
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ADAMTS13 is a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme is the von Willebrand Factor (vWF)-cleaving protease, which is responsible for cleaving at the site of Tyr842-Met843 of the vWF molecule. A deficiency of this enzyme is associated with thrombotic thrombocytopenic purpura.
Background Reference 1:
Zheng X., Chung D., Takayama T.K.J. Biol. Chem. 276:41059-41063(2001)
Background Reference 2:
Levy G.G., Nichols W.C.Nature 413:488-494(2001)
Background Reference 3:
Cal S., Obaya A.J., Llamazares M., Garabaya C.Gene 283:49-62(2002)
Background Reference 4:
Zheng X., Nishio K., Majerus E.M.J. Biol. Chem. 278:30136-30141(2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-911

List Size:
400 ul

List Price:

$495.00

Datasheet
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