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Western blot analysis of ADAP in K562 cell lysate with ADAP antibody at (A) 0.5 and (B) 1 μg/mL.
Immunocytochemistry of ADAP in K562 cells with ADAP antibody at 10 μg/mL.
Western blot analysis of ADAP in K562 cell lysate with ADAP antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

ADAP Antibody

CATALOG NUMBER: 4277

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
ADAP antibody was raised against a 20 amino acid synthetic peptide from near the carboxy terminus of human ADAP.
The immunogen is located within amino acids 640 - 690 of ADAP.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
ADAP antibody can be used for detection of ADAP by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Positive Control 2:
Cat. No. 17-004 - K-562 Cell Slide
Purification:
ADAP Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ADAP Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ADAP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FYB
Additional Names:
ADAP Antibody: ADAP, PRO0823, SLAP-130, SLAP130, FYN-binding protein, Adhesion and degranulation promoting adaptor protein, ADAP
Protein Accession Number:
NP_001456
Protein Gi Number:
42476118
Ncbi Gene Id Number:
2533
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ADAP Antibody: The adhesion and degranulation adaptor protein (ADAP) was initially identified as a molecular adapter that couples T cell receptor (TCR) stimulation to the avidity of integrins governing T cell adhesion. TCR stimulation promotes the formation of a multi-protein complex containing CARMA1, MALT1, and BCL-10, which through the association of ADAP, ultimately activates the NF-κB family of transcription factors. More recent experiments have shown that ADAP controls optimal T cell proliferation, cytokine production, and expression of the Bcl-2 family member Bcl-x(L), suggesting that ADAP regulates T cell activation by promoting antigen-dependent T cell-antigen presenting cell (APC) activation. At least three isoforms of ADAP are known to exist.
Background Reference 1:
Griffiths EK, Krawczyk C, Kong YY, et al. Positive regulation of T cell activation and integrin adhesion by the adapter Fyb/Slap. Science2001; 293:2260-3.
Background Reference 2:
Rawlings DJ, Sommer K, and Moreno-Garcia ME. The CARMA1 signalosome links the signalling machinery of adaptive and innate immunity in lymphocytes. Nat. Rev. Immunol.2006; 6:799-812.
Background Reference 3:
Medeiros RB, Burbach BJ, Mueller KL, et al. Regulation of NF-kappaB activation in T cells via association of the adapter proteins ADAP and CARMA1. Science2007; 316:754-8.
Background Reference 4:
Mueller KL, Thomas MS, Burbach BJ, et al. Adhesion and degranulation-promoting adapter protein (ADAP) positively regulates T cell sensitivity to antigen and T cell survival. J. Immunol.2007; 179:3559-69.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4277

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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