Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of extracts of various cell lines, using ADAR antibody (23-240) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 20s.
Immunohistochemistry of paraffin-embedded rat testis using ADAR antibody (23-240) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using ADAR antibody (23-240) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using ADAR antibody (23-240) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using ADAR antibody (23-240) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using ADAR antibody (23-240) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using ADAR antibody (23-240) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using ADAR antibody (23-240) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using ADAR antibody (23-240) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug SUZ12 antibody (23-183). Western blot was performed from the immunoprecipitate using SUZ12 antibody (23-183) at a dilition of 1:500.
Immunofluorescence analysis of HeLa cells using ADAR antibody (23-240) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using ADAR antibody (23-240) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 20s.

ADAR Antibody

CATALOG NUMBER: 23-240

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 150-250 of human ADAR (NP_001102.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
U-87MG
Positive Control 2:
A549
Positive Control 3:
DU145
Positive Control 4:
HeLa
Positive Control 5:
HepG2
Predicted Molecular Weight:
Observed: 136kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ADAR
Additional Names:
ADAR1, AGS6, DRADA, DSH, DSRAD, G1P1, IFI-4, IFI4, K88DSRBP, P136, double-stranded RNA-specific adenosine deaminase, 136 kDa double-stranded RNA-binding protein, adenosine deaminase acting on RNA 1-A, dsRNA adenosine deaminase, dsRNA adeonosine deaminase, interferon-induced protein 4, interferon-inducible protein 4
Ncbi Gene Id Number:
103
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-240

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369