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45-004 (1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-004 (1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ADH1 Antibody

CATALOG NUMBER: 45-004

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: STAGKVMKCKA
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 38kDa band observed in Human Liver lysates (calculated MW of 39.9kDa according to NP_000658.1, NP_000659.2 and 000660.1). Recommended concentration: 1-3ug/ml.Immunohistochemistry:Customer found this product to work in IHC on Human Liver.
Specificity:
This antibody is expected to recognise the alpha (ADH1A, NP_ 000658.1), the beta (ADH1B, NP_000659.2) and gamma (ADH1C, NP_000660.1) polypeptide variants of human alcohol dehydrogenase.
Positive Control 1:
Cat. No. 1304 - Human Liver Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ADH1A, B, C
Additional Names:
pan ADH, ADH1B, ADH2, alcohol dehydrogenase IB (class I), beta polypeptide, ADH, beta subunit, aldehyde reductase, alcohol dehydrogenase 2, alcohol, ADH, gamma subunit, alcohol dehydrogenase 3 (class I), gamma polypeptide dehydrogenase 2 (class I), beta polypeptide, alcohol dehydrogenase 1B (class I), beta polypeptide, ADH1C, alcohol dehydrogenase 1C (class I), gamma polypeptide, ADH3, aldehyde reductase, class I alcohol dehydrogenase, gamma subunit, ADH1A, alcohol dehydrogenase 1A (class I), alpha polypeptide, ADH1, ADH, alpha subunit, alcohol dehydrogenase 1 (class I), alpha polypeptide, aldehyde reductase, class I alcohol dehydrogenase, alpha subunit
Protein Accession Number:
NP_000658.1,NP_000659.2,NP_000660.1
Protein Gi Number:
4501929
Ncbi Gene Id Number:
124;125;126
Background Reference 1:
Ikuta T, Fujiyoshi T, Kurachi K, Yoshida A. Molecular cloning of a full-length cDNA for human alcohol dehydrogenase. Proc Natl Acad Sci USA. 1985 May;82(9):2703-7.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-004

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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