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Western blot analysis in mouse heart tissue lysates (35ug/lane).
Western blot analysis of ADH4 using rabbit polyclonal ADH4 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ADH4 gene.
ADH4 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in mouse heart tissue lysates (35ug/lane).

ADH4 Antibody

CATALOG NUMBER: 55-047

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This ADH4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 319-348 amino acids from the C-terminal region of human ADH4.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
40 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ADH4
Additional Names:
Alcohol dehydrogenase 4, Alcohol dehydrogenase class II pi chain, ADH4
Protein Accession Number:
P08319
Protein Gi Number:
308153684
Ncbi Gene Id Number:
127
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes class II alcohol dehydrogenase 4 pisubunit, which is a member of the alcohol dehydrogenase family.Members of this enzyme family metabolize a wide variety ofsubstrates, including ethanol, retinol, other aliphatic alcohols,hydroxysteroids, and lipid peroxidation products. Class II alcoholdehydrogenase is a homodimer composed of 2 pi subunits. It exhibitsa high activity for oxidation of long-chain aliphatic alcohols andaromatic alcohols and is less sensitive to pyrazole. This gene islocalized to chromosome 4 in the cluster of alcohol dehydrogenasegenes.
Background Reference 1:
Pochareddy, S., et al. Gene 460 (1-2), 1-7 (2010) :
Background Reference 2:
Preuss, U.W., et al. Addict Biol (2010) In press :
Background Reference 3:
Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010)
Background Reference 4:
Rainero, I., et al. Headache 50(1):92-98(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-047

List Size:
400 ul

List Price:

$495.00

Datasheet
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