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Western blot analysis of ADH6 Antibody in Hela cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ADH6 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of ADH6 Antibody in Hela cell line lysates (35ug/lane)

ADH6 Antibody

CATALOG NUMBER: 63-836

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ADH6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 209-240 amino acids from the Central region of human ADH6.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
39 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ADH6
Additional Names:
Alcohol dehydrogenase 6, ADH6
Protein Accession Number:
P28332
Protein Gi Number:
254763246
Ncbi Gene Id Number:
130
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ADH6 encodes class V alcohol dehydrogenase, which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This protein is expressed in the stomach as well as in the liver, and it contains a glucocorticoid response element upstream of its 5' UTR, which is a steroid hormone receptor binding site.
Background Reference 1:
Cui,R., et.al., Gastroenterology 137 (5), 1768-1775 (2009)
Background Reference 2:
Saito,A., et.al., J. Hum. Genet. 54 (6), 317-323 (2009)
Background Reference 3:
Tabakoff,B., et.al., BMC Biol. 7, 70 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-836

List Size:
400 ul

List Price:

$495.00

Datasheet
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