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ADH7 Antibody

CATALOG NUMBER: 45-208

Clonality:
Polyclonal
Tested Applications:
ELISA
Host Species:
Goat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse
Immunogen:
The immunogen for this antibody is: CISPKDSTKPISE
Conjugate:
Unconjugated
Tested Applications:
ELISA
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 26kDa band in lysates of Human Duodenum and Liver and of Rat stomach after 0.2ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 41.5kDa according to NP_000664.2. The 26kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ADH7
Additional Names:
ADH7, alcohol dehydrogenase 7 (class IV), mu or sigma polypeptide, ADH-4 , alcohol dehydrogenase-7, class IV alcohol dehydrogenase 7 mu or sigma subunit, gastric alcohol dehydrogenase.
Protein Accession Number:
NP_000664.2
Protein Gi Number:
71743840
Ncbi Gene Id Number:
131
Background Reference 1:
Zgombic-Knight M, Foglio MH, Duester G. Genomic structure and expression of the ADH7 gene encoding human class IV alcohol dehydrogenase, the form most efficient for retinol metabolism in vitro. J Biol Chem. 1995 Mar 3;270(9):4305-11.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-208

List Size:
0.1 mg

List Price:

$391.00

Datasheet
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