Adiponectin Antibody [1.3-13A05-2D09]

Application Note:

ELISA:
Sandwich:
In a sandwich ELISA (assuming 100μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant Human Adiponectin when used in conjunction with compatible detection reagents at a concentration of approximately 0.5-1.0 μg/mL.
Western Blot:
To detect Human Adiponectin by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human Adiponectin is 0.5-1.0 ng/lane, under reducing conditions and 1.0-2.0 ng/lane, under non-reducing conditions.