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Western blot analysis in mouse kidney tissue lysates (35ug/lane).
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in mouse kidney tissue lysates (35ug/lane).

ADO Antibody

CATALOG NUMBER: 55-611

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This ADO antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 206-233 amino acids from the C-terminal region of human ADO.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
30 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ADO
Additional Names:
2-aminoethanethiol dioxygenase, Cysteamine dioxygenase, ADO, C10orf22
Protein Accession Number:
Q96SZ5
Protein Gi Number:
88984104
Ncbi Gene Id Number:
84890
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Human thiol dioxygenases include cysteine dioxygenase(CDO; MIM 603943) and cysteamine (2-aminoethanethiol) dioxygenase(ADO; EC 1.13.11.19). CDO adds 2 oxygen atoms to free cysteine,whereas ADO adds 2 oxygen atoms to free cysteamine to formhypotaurine (Dominy et al., 2007 [PubMed 17581819]).[supplied byOMIM].
Background Reference 1:
Dominy, J.E. Jr., et al. J. Biol. Chem. 282(35):25189-25198(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-611

List Size:
400 ul

List Price:

$495.00

Datasheet
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