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Western blot analysis of ADORA2B in mouse kidney tissue lysate with ADORA2B antibody at (A) 0.25 and (B) 0.5 μg/mL
Immunohistochemistry of ADORA2B in rat colon tissue with ADORA2B antibody at 5 μg/mL.
Immunofluorescence of ADORA2B in rat colon tissue with ADORA2B antibody at 20 μg/mL.
Western blot analysis of ADORA2B in mouse kidney tissue lysate with ADORA2B antibody at (A) 0.25 and (B) 0.5 μg/mL
Trial Size

ADORA2B Antibody

CATALOG NUMBER: 8111

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Rabbit: (80%)
Immunogen:
ADORA2B antibody was raised against a 19 amino acid peptide near the carboxy terminus of human ADORA2B.
The immunogen is located within the last 50 amino acids of ADORA2B.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
ADORA2B antibody can be used for detection of ADORA2B by Western blot at 0.25 – 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Specificity:
ADORA2B antibody is human, mouse and rat reactive. At least two isoforms of ADORA2B are known to exist; this antibody will detect both isoforms. ADORA2B antibody is predicted to not cross-react with ADORA2A.
Positive Control 1:
Cat. No. 1405 - Mouse Kidney Tissue Lysate
Positive Control 2:
Cat. No. 1411 - Mouse Colon Tissue Lysate
Predicted Molecular Weight:
Predicted: 37 kDa
Observed: 41 kDa
Purification:
ADORA2B antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ADORA2B antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ADORA2B antibody can be stored at 4°C for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ADORA2B
Additional Names:
Adenosine receptor A2b, ADORA2
Protein Accession Number:
NP_000667
Protein Gi Number:
4501951
Ncbi Gene Id Number:
136
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The adenosine receptor ADORA2B is a member of the G protein-coupled receptor superfamily and is an integral membrane protein that stimulates adenylate cyclase activity in the presence of adenosine (1,2). Extracellular adenosine triggers a potent anti-inflammatory response that is mediated in part by ADORA2B including the stimulation of IL-10 production (3-5). Activation of ADORA2B can also enhance the abundance of regulatory T cells (Tregs), a class of cells that work to constrain inflammation (6).
Background Reference 1:
Pierce KD, Furlong TJ, Selbie LA, et al. Molecular cloning and expression of an adenosine A2b receptor from human brain. Biochem. Biophys. Res. Commun. 1992; 187:86-93.
Background Reference 2:
Thimm D, Schiedel AC, Sherbiny FF, et al. Ligand-specific binding and activation of the human adenosine A(2B) receptor. Biochem. 2013; 52:726-40.
Background Reference 3:
Blackburn MR, Vance CO, Morschl E, et al. Adenosine receptors and inflammation. Handb. Exp. Pharmacol. 2009; 215-69.
Background Reference 4:
Grenz A, Homann D, Eltzschig HK. Extracellular adenosine: a safety signal that dampens hypoxia-induced inflammation during ischemia. Antioxid. Redox Signal. 2011; 15:2221-34.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8111

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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