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Western blot analysis of ADRA1B Antibody in NCI-H460 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain with ADRA1B Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of ADRA1B Antibody in NCI-H460 cell line lysates (35ug/lane)

ADRA1B Antibody

CATALOG NUMBER: 63-929

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rabbit, Rat
Immunogen:
This ADRA1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 270-297 amino acids from the Central region of human ADRA1B.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
57 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ADRA1B
Additional Names:
Alpha-1B adrenergic receptor, Alpha-1B adrenoreceptor, Alpha-1B adrenoceptor, ADRA1B
Protein Accession Number:
P35368
Protein Gi Number:
116241241
Ncbi Gene Id Number:
147
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This protein encodes alpha-1B-adrenergic receptor, which induces neoplastic transformation when transfected into NIH 3T3 fibroblasts and other cell lines. Thus, this normal cellular gene is identified as a protooncogene. This protein comprises 2 exons and a single large intron of at least 20 kb that interrupts the coding region.
Background Reference 1:
Mathias,R.A., J. Allergy Clin. Immunol. 125 (2), 336-346 (2010)
Background Reference 2:
Jensen,B.C., Circ Heart Fail(6), 654-663 (2009)
Background Reference 3:
Gratacos,M., Am. J. Med. Genet.Neuropsychiatr. Genet. 150B (6), 808-816 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-929

List Size:
400 ul

List Price:

$495.00

Datasheet
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