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Immunohistochemical analysis of paraffin-embedded H. kidney section using ADRA1D Antibody (N-term). Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Flow cytometric analysis of MCF-7 cells using ADRA1D Antibody (N-term)(green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded H. small intestine section using ADRA1D Antibody (N-term). Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded R. kidney section using ADRA1D Antibody (N-term). Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis of lysates from A549, LNCaP, PC-3 cell line and rat brain tissue lysate (from left to right), using ADRA1D Antibody at 1:1000 at each lane.
Immunohistochemical analysis of paraffin-embedded H. kidney section using ADRA1D Antibody (N-term). Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

ADRA1D Antibody

CATALOG NUMBER: 61-046

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Bovine, Dog, Rabbit, Sheep
Immunogen:
This ADRA1D antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1-30amino acids from the N-terminal region of human ADRA1D.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For IHC-P starting dilution is: 1:25
For FACS starting dilution is: 1:25
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
60 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ADRA1D
Additional Names:
Alpha-1D adrenergic receptor, Alpha-1A adrenergic receptor, Alpha-1D adrenoreceptor, Alpha-1D adrenoceptor, Alpha-adrenergic receptor 1a, ADRA1D, ADRA1A
Protein Accession Number:
P25100
Protein Gi Number:
1168243
Ncbi Gene Id Number:
146
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This alpha-adrenergic receptor mediates its effect through the influx of extracellular calcium.
Background Reference 1:
Bruno J.F.,et al.Biochem. Biophys. Res. Commun. 179:1485-1490(1991).
Background Reference 2:
Forray C.,et al.Mol. Pharmacol. 45:703-708(1994).
Background Reference 3:
Schwinn D.A.,et al.J. Pharmacol. Exp. Ther. 272:134-142(1995).
Background Reference 4:
Weinberg D.H.,et al.Biochem. Biophys. Res. Commun. 201:1296-1304(1994).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-046

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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