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Western blot analysis of extracts of various cell lines, using ADRBK1 antibody (16-681) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts from normal (control) and ADRBK1 knockout (KO) 293T cells, using ADRBK1 antibody (16-681) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat brain using ADRBK1 Antibody (16-681) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using ADRBK1 Antibody (16-681) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using ADRBK1 Antibody (16-681) at dilution of 1:200 (40x lens).
Western blot analysis of extracts of various cell lines, using ADRBK1 antibody (16-681) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
KO

ADRBK1 Antibody, KO Validated

CATALOG NUMBER: 16-681

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 440-689 of human ADRBK1 (NP_001610.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
HT-29
Positive Control 2:
Mouse spleen
Positive Control 3:
Mouse brain
Predicted Molecular Weight:
Observed: 80kDa
Validation:
Antibody is Knockout validated.
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ADRBK1
Additional Names:
ADRBK1, adrenergic, beta, receptor kinase 1, BARK1, BETA-ARK1, GRK2, beta adrenergic receptor kinase 1, BARK
Ncbi Gene Id Number:
156
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-681

List Size:
100 uL

List Price:

$540.00

Datasheet
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