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Western blot analysis of ADRM1 in EL cell lysate with ADRM1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of ADRM1 in human spleen tissue with ADRM1 antibody at 5 μg/mL.
Western blot analysis of ADRM1 in EL cell lysate with ADRM1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

ADRM1 Antibody

CATALOG NUMBER: 8119

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
ADRM1 antibody was raised against a 19 amino acid peptide near the center of human ADRM1.
The immunogen is located within amino acids 210 - 260 of ADRM1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
ADRM1 antibody can be used for detection of ADRM1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Specificity:
ADRM1 antibody is human, mouse and rat reactive. At least two isoforms of ADRM1 are know to exist; this antibody will detect both isoforms.
Positive Control 1:
Cat. No. 1287 - EL4 Cell Lysate
Positive Control 2:
Cat. No. 10-901 - Human Spleen Tissue Slide
Predicted Molecular Weight:
Predicted: 45 kDa
Observed: 48 kDa
Purification:
ADRM1 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ADRM1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ADRM1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
ADRM1
Additional Names:
Adhesion regulating molecule 1, ARM1, Gp110, hRpn13, Proteasomal ubiquitin receptor ADRM1, Proteasome regulatory particle non-ATPase 13, Rpn13
Protein Accession Number:
NP_783163
Protein Gi Number:
28373194
Ncbi Gene Id Number:
11047
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The adhesion-regulating molecule 1 (ADRM1) is thought to be involved in protein recruitment and cell adhesion and functions as a proteasomal ubiquitin receptor (1). ADRM1 promotes the activity of UCH-L5 and plays a role in lymphocyte homing. The carboxy-terminal domain of mammalian ADRM1 serves to bind and enhance the isopeptidase activity of UCHL5/UCH37, perhaps serving as a mechanism to accelerate ubiquitin chain disassembly (2,3). ADRM1 expression is induced by IFN-gamma in some cancer cell lines and its expression is upregulated in other metastatic cells, suggesting a role in carcinogenesis (4,5).
Background Reference 1:
Husnjak K, Elsasser S, Zhang N, et al. Proteasome subunit Rpn13 is a novel ubiquitin receptor. Nature 2008; 453:481-8.
Background Reference 2:
Yao T, Song L, Xu W, et al. Proteasome recruitment and activation of the Uch37 deubiquitinating enzyme by Adrm1. Nat. Cell Biol. 2006; 8:994-1002.
Background Reference 3:
Qiu XB, Ouyang SY, Li CJ, et al. hRpn13/ADRM1/GP110 is a novel proteasome subunit that binds the deubiquitinating enzyme, UCH37. EMBO J. 2006; 25:5742-53.
Background Reference 4:
Al-Shami A, Jhaver KG, Vogel P, et al. Regulators of the proteasome pathway, Uch37 and Rpn13, play distinct roles in mouse development. PLoS One 2010; 5:e13654.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8119

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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