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Western blot analysis of ADROPIN in human brain tissue lysate with ADROPIN antibody at 2 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of ADROPIN in human brain tissue with ADROPIN antibody at 5 μg/mL.
Immunofluorescence of ADROPIN in human brain tissue with ADROPIN antibody at 20 μg/mL.
Western blot analysis of ADROPIN in human brain tissue lysate with ADROPIN antibody at 2 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

ADROPIN Antibody

CATALOG NUMBER: 7527

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Immunogen:
ADROPIN antibody was raised against an 18 amino acid peptide near the carboxy terminus of human ADROPIN.
The immunogen is located within the last 50 amino acids of ADROPIN.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
ADROPIN antibody can be used for detection of ADROPIN by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
ADROPIN antibody is human, mouse and rat reactive. Despite its predicted molecular weight, ADROPIN often migrates at a higher than expected molecular weight in SDS-PAGE.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Predicted Molecular Weight:
Predicted: 8 kDa
Observed: 16 kDa
Purification:
ADROPIN antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ADROPIN antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures
Ncbi Official Symbol:
ENHO
Additional Names:
ADROPIN Antibody: UNQ470, C9orf165, UNQ470/PRO830, Adropin, Energy homeostasis-associated protein
Protein Accession Number:
NP_940975
Protein Gi Number:
58218977
Ncbi Gene Id Number:
375704
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ADROPIN is a recently identified protein that has been implicated in the maintenance of energy homeostasis and insulin resistance (1-3). ADROPIN expression is regulated by energy status and dietary nutrient content and is altered by obesity and regulates the expression of hepatic lipogenic genes and adipose tissue peroxisome proliferator-activated receptor gamma (PPAR-gamma) (1). ADROPIN levels increase with dietary fat content (2). ADROPIN has also been proposed to play a role in the regulation of endothelial function (3).
Background Reference 1:
Kumar KG, Trevaskis JL, Lam DD, et al. Identification of Adropin as a secreted factor linking dietary macronutrient intake with energy homeostasis and lipid metabolism. Cell Metab. 2008; 8:468-81.
Background Reference 2:
Kumar KG, Zhang J, Gao S, et al. Adropin deficiency is associated with increased adiposity and insulin resistance. Obesity 2012; 1394-402.
Background Reference 3:
Lovren F, Pan Y, Quan A, et al. Adropin is a novel regulator of endothelial function. Circulation 2010; 122:S185-92.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7527

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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