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Western blot analysis of extracts of various cell lines, using ADSS Antibody (14-399) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human lung cancer using ADSS antibody (14-399) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using ADSS antibody (14-399) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using ADSS antibody (14-399) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using ADSS antibody (14-399) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using ADSS antibody (14-399). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using ADSS Antibody (14-399) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

ADSS Antibody

CATALOG NUMBER: 14-399

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ADSS (NP_001117.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:100
Positive Control 1:
293T
Positive Control 2:
Mouse lung
Positive Control 3:
Mouse brain
Predicted Molecular Weight:
Observed: 50kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ADSS
Additional Names:
Adenylosuccinate synthetase isozyme 2 {ECO:0000255, HAMAP-Rule:MF_03127}, AMPSase 2 {ECO:0000255, HAMAP-Rule:MF_03127}, AdSS 2 {ECO:0000255, HAMAP-Rule:MF_03127}, 6344 {ECO:0000255, HAMAP-Rule:MF_03127}, Adenylosuccinate synthetase, acidic isozyme {ECO:0000255, HAMAP-Rule:MF_03127}, Adenylosuccinate synthetase, liver isozyme {ECO:0000255, HAMAP-Rule:MF_03127}, L-type adenylosuccinate synthetase {ECO:0000255, HAMAP-Rule:MF_03127}, IMP--aspartate ligase 2 {ECO:0000255, HAMAP-Rule:MF_03127}, ADSS {ECO:0000255, HAMAP-Rule:MF_03127}
Ncbi Gene Id Number:
159
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes the enzyme adenylosuccinate synthetase which catalyzes the first committed step in the conversion of inosine monophosphate to adenosine monophosphate. A pseudogene of this gene is found on chromosome 17.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-399

List Size:
100 uL

List Price:

$436.00

Out of stock

Datasheet
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